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| Registros recuperados: 13 | |
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| Limeira,Daniel M.; Renesto,Erasmo; Zawadzki,Cláudio H.. |
| Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only loci Aat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Allozymes; Fish genetics; Genetic distance; Polymorphism; Loricariidae; Pisces. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200034 |
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| Zawadzki,Cláudio H.; Renesto,Erasmo; Peres,Maria Dolores; Paiva,Suzana. |
| Three Brazilian populations of the armored catfish Hypostomus regani (Ihering, 1905) were sampled, one from the Corumbá Reservoir in Goiás state, another from the Itaipu Reservoir in Paraná state and a third from the Manso Reservoir in Mato Grosso state. Allozyme electrophoresis was used to establish the genetic structure of the species, with the analysis of liver, heart and muscles tissues allowing the scoring of 25 loci from 14 enzymatic systems. Although no diagnostic loci were found, some exclusive rare alleles were recorded for the three populations. The genetically most similar populations were those from Corumbá and Itaipu, and the most distant were the populations from Manso and Corumbá. The allozyme data showed three structured populations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Genetic variability; Hypostomus regani; Loricariidae; Paraguay and Paraná Rivers. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400025 |
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| Ito,Kennya F.; Renesto,Erasmo; Zawadzki,Cláudio H.. |
| Two syntopic morphotypes of the genus Hypostomus - H. nigromaculatus and H. cf. nigromaculatus (Atlântico Stream, Paraná State) - were compared through the allozyme electrophoresis technique. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, attributing the score of 20 loci, with a total of 30 alleles. Six loci were diagnostic (Aat-2, Gcdh-1, Gpi-A, Idh-1, Ldh-A and Mdh-A), indicating the presence of interjacent reproductive isolation. The occurrence of few polymorphic loci acknowledge two morphotypes, with heterozygosity values He = 0.0291 for H. nigromaculatus and He = 0.0346 for H. cf. nigromaculatus. F IS statistics demonstrated fixation of the alleles in the two morphotypes. Genetic identity (I)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Hypostomus nigromaculatus; Fish genetics; Genetic distance; Polymorphism. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100008 |
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| Signorini,Tiago; Renesto,Erasmo; Machado,Maria de Fátima PS; Bespalhok,Danielle das N; Monteiro,Eliane R. |
| O gênero Capsicum compreende um rico gênero em aspectos morfológicos e genéticos. Estudos de diversidade genética ligada a este gênero são necessários para maior compreensão sobre as espécies, além de descobertas sobre novas informações em bancos de germoplasma de Capsicum. Este trabalho teve como objetivo analisar a variabilidade genética de três espécies do Capsicum (9 acessos) oriundas do Banco Ativo de Germoplasma da Universidade Federal do Piauí (BAGC). Para isso, utilizou-se da técnica de eletroforese de isoenzimas em gel de amido para a análise de cinco sistemas isoenzimáticos (ACP, GPI, IDH, MDH e PGM). Foram detectados sete loci enzimáticos e 13 alelos, sendo que cinco alelos foram exclusivos, três presentes na população de C. annuum var.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aloenzima; Variabilidade genética. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000400005 |
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| Renesto,Erasmo; Zawadzki,Cláudio Henrique; Revaldaves,Eloísa. |
| The existence of reproductive isolation between two morphs of catfish, endemic to the Iguaçu River (Brazil), was examined by enzyme starch gel electrophoresis. Tissues of 19 catfish (Pimelodus ortmanni) and 15 of a similar morph (Pimelodus sp.), which differs from P. ortmanni by presenting larger and more scattered dusky spots on its skin, were analyzed. A Nei's (1978) genetic identity of 0.551 was determined by the analysis of 22 enzyme loci. The loci EST*1, EST*2, GDH*1, GPI*1, GPI*2, IDH*1, MDH*1, MDH*2, and PGM*1 were fixed for different alleles in each morph, that is, no heterozygote was found for these loci. The enzymatic patterns observed for the two morphs indicate both that the taxa are reproductively isolated and that they in fact represent... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400017 |
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| Peres,Maria Dolores; Vasconcelos,Marcio dos Santos; Renesto,Erasmo. |
| Allozyme data was used to assess the genetic diversity Astyanax altiparanae populations from the floodplain of the Upper Paraná River (PR). Specimens were collected in the southern Brazilian state of Paraná from PR in Porto Rico municipality and Ribeirão Ficha (RF) in Ubiratã municipality. The authors used 15% (w/v) corn starch gel electrophoresis to identify 21 putative loci for 13 enzymatic systems: Aspartate aminotransferase, 2.6.1.1 (AAT), Acid phosphatase, 3.1.3.2 (ACP), Esterase, 3.1.1.1 (EST), Glycerol-3-phosphate dehydrogenase, 1.1.1.8 (G3PDH), Glucose-6-phosphate dehydrogenase, 1.1.1.49 (G6PDH), Glucose-6-phosphate isomerase, 5.3.1.9 (GPI), Iditol dehydrogenase, 1.1.1.14 (IDDH), Isocitrate dehydrogenase - NADP+, 1.1.1.42 (IDH), L-Lactate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Astyanax; Fishes; Genetic variability; Paraná River floodplain. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500011 |
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| Philippsen,Juliana S.; Renesto,Erasmo; Gealh,Ana Maria; Artoni,Roberto F.; Shibatta,Oscar A.; Zawadzki,Claudio H.. |
| Four samples of Neoplecostomus yapo were analyzed through the allozyme electrophoresis technique in corn starch gel. The allozyme pattern was similar to those found in N. paranensis with 24 loci scored. Two samples (ribeirão Atlântico and ribeirão Uraí) showed monomorphic bands for all 24 loci, whereas the other two (rio Verde and rio Fortaleza) showed 8.3% of polymorphic loci. The He genetic variability estimates for the rios Verde and Fortaleza populations were 0.0195 and 0.0179, respectively, too much inferior to the mean heterozygosity summed to species from the whole world (0.051). The Wright statistical values F IS = 0.5181, F IT = 0.5681 and F ST = 0.1039 and the genetic distance of Nei values showed that the four samples are genetically very... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Genetic variation; Neoplecostominae; Neotropical fishes; Siluriformes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000100004 |
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| Rezende,Judy Ruiz; Renesto,Erasmo; Zawadzki,Cláudio Henrique. |
| The genetic variability of three Gymnotus species from the Caracu stream, a small tributary of the left margin of Paraná River (Brazilian upper Paraná River floodplain), was estimated with data of 17 putative allozyme loci, which were obtained by using corn starch gel electrophoresis of 10 enzymatic systems: Aspartate aminotransferase (E. C. 2.6.1.1), Alcohol dehydrogenase (E. C. 1.1.1.1), Esterase (E. C. 3.1.1.1), Glucose dehydrogenase (E. C. 1.1.1.118), Glycerol-3-phosphate dehydrogenase (E. C. 1.1.1.8), Isocitrate dehydrogenase (E. C. 1.1.1.42), L-Lactate dehydrogenase (E. C. 1.1.1.27), Malate dehydrogenase (E. C. 1.1.1.37), Superoxide dismutase (E. C. 1.15.1.1) and Sorbitol dehydrogenase (E. C. 1.1.1.14). The genetic diversity was estimated as He =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymorphism; Genetic distance; Allozyme; Heterozygosity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400010 |
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| Paiva,Suzana de; Renesto,Erasmo; Zawadzki,Cláudio Henrique. |
| Hypostomus strigaticeps and two morphotypes of Hypostomus were collected from Ribeirão Maringá, a small tributary of the Rio Pirapó, an effluent of the upper Rio Paraná. The three populations were analyzed by allozyme electrophoresis that allowed the scoring of 25 loci from 14 enzyme systems. Heterozygosity values (He) were 0.028 in H. strigaticeps, 0.027 in Hypostomus sp. 1 and zero in Hypostomus sp. 2. Several diagnostic loci and fixed differences were observed for each population at loci Acp-A, Gcdh-A and Mdhp-A. Thus, all populations were genetically distinct, although there were many common alleles. The unbiased genetic identities of Nei (I) were estimated as 0.780 for Hypostomus sp. 1 and H. strigaticeps, 0.357 for H. strigaticeps and Hypostomus sp.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allozymes; Heterozygosity; Hypostomus; Loricariidae; Systematic. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300005 |
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| Revaldaves,Eloísa; Renesto,Erasmo; Machado,Maria F.P.S.. |
| The genetic variability of the "curimba", Prochilodus lineatus, from three locations in the Paraná river basin, was investigated by starch gel electrophoresis. A total of 160 specimens were analyzed for 19 enzymes, 12 of which permitted successful interpretation of electrophoretic patterns. Eighteen loci were identified and six of them proved to be polymorphic (EST-1*, EST-2*, IDH-1*, PGM-1*, PGM-2*, LDH-2*). Mean heterozygosity was considered high (13%) by comparison with the literature. A low level of differentiation was found among subpopulations, with mean F ST = 0.018. Values of genetic distance and genetic identity suggest that, at least along this stretch of the river, P. lineatus comprises a single breed with high gene flow. This analysis has... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000300005 |
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| Lassala,Maria Dolores Peres; Renesto,Erasmo. |
| We estimated the genetic variability of nine fish species from the Brazilian upper Paraná River floodplain (Astyanax altiparanae, Hoplias malabaricus, Leporinus lacustris, Loricariichthys platymetopon, Parauchenipterus galeatus, Pimelodus maculatus, Rhaphiodon vulpinus, Roeboides paranensis and Serrasalmus marginatus) based on data for 36 putative allozyme loci obtained using corn starch gel electrophoresis of 13 enzymatic systems: aspartate aminotransferase (EC 2.6.1.1), acid phosphatase (EC 3.1.3.2), esterase (EC 3.1.1.1), glycerol-3-phosphate dehydrogenase (EC 1.1.1.8), glucose-6-phosphate dehydrogenase (EC 1.1.1.49), glucose-6-phosphate isomerase (EC 5.3.1.9), Iditol dehydrogenase (EC 1.1.1.14), isocitrate dehydrogenase - NADP+ (EC 1.1.1.42), L-lactate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Heterozygosity; Paraná River; Polymorphism; Reproductive strategies; Tropical fish. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400030 |
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| Registros recuperados: 13 | |
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